Bacterial L-forms on tap: an improved methodology to generate Bacillus subtilis L-forms heralds a new era of research
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Bacterial L-forms on tap: an improved methodology to generate Bacillus subtilis L-forms heralds a new era of research.
Bacterial L-forms are cell wall-less forms of bacteria that usually grow with a conventional cell wall. Despite being important for research, L-forms are difficult to generate reproducibly and research in this area is challenging. Domínguez-Cuevas et al. (2011) report a method to rapidly, quantitatively and reproducibly generate populations of L-forms in Bacillus subtilis. Importantly, the meth...
متن کاملAlterations of the L-forms of a sporebearing bacillus.
A large pleomorphic gram-negative bacillus developed as a contaminant on blood-agar. Spores were formed in one culture. L-forms were produced with penicillin on blood-agar with 2.5% NaCl; they grew well when transplanted to agar with 0.5% NaCl. After several transplants and long incubation of the L-forms without penicillin, in three transplants small gram-negative pleomorphic bacilli grew, but ...
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L-forms of Bacillus subtilis can be isolated by treatment of the parent strain sequentially with N-methyl-N'-nitro-N-nitrosoguanidine and lysozyme and selection of the surviving protoplasts on semisolid medium containing 2,000 units of penicillin per ml. Some of these clones can be adapted to grow in liquid cultures containing 1.2 m NaCl. This method will aid in the isolation of cell wall mutan...
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Numerous morphological, physiological, and biochemical studies have been carried out on bacterial forms exhibiting a pronounced osmotic fragility (for review see Weibull, 1958b). Naked bacterial protoplasts, obtained by means of a complete removal of the cell wall by lysozyme in a sucrose medium (Weibull, 1953) offer an example of such an osmotically fragile structure. Osmotically sensitive for...
متن کاملFever of unknown origin: attempts to isolate L-forms and other aberrant bacterial forms.
An investigation was conducted with 65 selected febrile patients, 33 of whom fulfilled in all respects the classic criteria of "fever of unknown origin." Clinical evaluation included extensive radiological and immunological testing. Multiple blood cultures were examined by conventional methods in control studies. In addition, venous blood was cultured in a variety of hyperosmolar media using th...
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ژورنال
عنوان ژورنال: Molecular Microbiology
سال: 2011
ISSN: 0950-382X
DOI: 10.1111/j.1365-2958.2011.07922.x